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Victor Edson Rogerio

Universidade Federal de Sao Paulo, Brazil

Title: The tooth as a monitor of cholestatic liver disease in rats

Biography

Biography: Victor Edson Rogerio

Abstract

Statement of the Problem: The cholestatic liver disease (CLD) leads to systemic metabolic consequences, causing comorbidities of difficult treatment and high mortality. If it does not have an early diagnostic, the only chance of patient’s survival is liver transplantation. The progression of a persistent CLD is associated to clotting disorders and increasing of the infections risk. These patients are usually subjected to multiple blood collections by venipuncture to monitor the progression of their liver disease, but these morbidities can become an ordinary blood collection, by venipuncture, very threatening to these patients. CLD patients’ have shown greenish tooth due to the intrinsic and progressive deposition of bilirubin, which is a porphyrin. In those circumstances, if it were possible to correlate the deposition of bilirubin with progression of the disease by a noninvasive method, that would be of a great value in the clinical management of the CLD patients.

Methodology & Theoretical Orientation: After confirming the capacity of DIAGNOdent to measure varying bile concentrations in plaster blocks, a cholestatic liver disease model was studied to detect increasing bilirubin impregnation in the teeth of rats. Wistar-EPM rats (n ¼ 50) were divided into three groups: (1) BDL: rats submitted to bile duct ligation (BDL); (2) Naıve: rats without procedure; and (3) Sham: rats submitted to laparotomy without BDL (n ¼ 10/period/group). The rats’ teeth were monitored with the use of DIAGNOdent

before the procedure and at days 10 and 50 following surgery. Serum bilirubin was also monitored.

Findings: Tests in vitro showed that DIAGNOdent detected bile in plaster blocks according to its concentration. BDL promoted progressive liver dysfunction, with death occurring approximately 50 days later. DIAGNOdent values obtained on teeth showed correlation with the progression of serum hyperbilirubinemia.

Conclusion & Significance: The tooth was found to be a good tissue for noninvasively monitoring the progression of bilirubinemia in cholestatic liver disease in rats by using DIAGNOdent.